TABLE 1

Kinetic parameters of YM155

The kinetic parameters of YM155 uptake in hSLC35F2-HEK293 and mSLC35F2-HEK293 cells (Supplemental Fig. 1D) and parental hCMEC/D3 cells (Fig. 2B). The values represent mean ± computer-calculated S.D. The experiments were repeated at least twice.

Km, appVmax, appPdif
μMpmol/min per milligram proteinμl/min per milligram protein
hSLC35F20.310 ± 0.03815.2 ± 1.12.41 ± 0.21
mSLC35F21.55 ± 0.3697.1 ± 20.92.47 ± 1.68
hCMEC/D30.573 ± 0.1661.74 ± 0.350.277 ± 0.046