TABLE 2

Effects of organic cations on YM155 uptake in PC-3 and hCMEC/D3 cells

We determined 5 min YM155 (0.1 μM) uptake mediated by SLC35F2 in control PC-3 or hCMEC/D3 cells in the absence and presence of the test compounds. Values represent mean ± S.E. (n = 3 to 4). N.D. indicates no data. The experiments were repeated at least twice.

Conditions/CompoundsConc.Control YM155 Uptake (%)
PC-3hCMEC/D3
ControlFree100 ± 6100 ± 8
SLC35F2 knockout10.3 ± 1.0**N.D.
l-carnitine1 mM125 ± 14**120 ± 4*
TEA83.4 ± 1.864.2 ± 4.9**
Varenicline76.4 ± 3.0**84.3 ± 10.4
Famotidine16.4 ± 1.8**15.1 ± 0.6**
Tramadol13.0 ± 1.0**32.6 ± 5.6**
Clonidine9.06 ± 0.47**21.9 ± 1.3**
Diphenhydramine5.65 ± 0.32**12.4 ± 0.7**
Pyrilamine5.46 ± 0.97**14.1 ± 1.3**
Imipramine5.36 ± 0.34**34.4 ± 2.5**
Verapamil100 μM5.11 ± 0.28**27.0 ± 6.1**
Quinidine3.22 ± 0.51**15.1 ± 0.6**
  • * P < 0.05; **P < 0.01 vs. control.