Peptide sequences and MS parameters for AOX and ALDH1A signature peptides selected for protein quantification using LC-MS/MS
Labeled amino acids in the SIL-peptides are shown as italicized letters. SIL-peptides of AOX and ALDH1A1 were labeled with [13C615N2]arginine and [13C615N2]lysine, respectively. ALDH1A2 and ALDH1A3 were not detected in any HLS9 samples used in this study.
| Peptide | Precursor Ion (m/z) | Fragment Ion (m/z) | DP (V) | CE (V) | |
|---|---|---|---|---|---|
| AOX | VFFGEGDGIIR | 605.3 | 963.5, 816.4 | 80 | 17 |
| VFFGEGDGIIR | 610.3 | 973.5, 826.4 | 80 | 17 | |
| ALDH1A1 | ANNTFYGLSAGVFTK | 795.4 | 1042.6, 879.5 | 120 | 20 |
| ANNTFYGLSAGVFTK | 799.4 | 1050.6, 887.5 | 120 | 20 | |
| ALDH1A2 | ILELIQSGVAEGAK | 714.4 | 846.4, 959.5 | 120 | 30 |
| ALDH1A3 | EEIFGPVQPILK | 685.4 | 851.5, 794.5 | 81 | 34 |
CE, collision energy; DP, declustering potential; V, voltage.