Primers used to introduce mutations in the AADAC gene and for AADAC genotyping
The underlined letters indicate mutations.
| Mutation | Direction | Sequence (5′→3′) |
|---|---|---|
| Primers used to introduce mutations | ||
| T74A | Sense | CCCACCAGCCTCAGATGAAAATGTCACTGTGAT |
| Antisense | TCTGAGGCTGGTGGGACTTCATCAAAGCTCCCG | |
| V172I | Sense | TAAAAAAATTCTTGCAAAATATGGTGTGAACCC |
| Antisense | GCAAGAATTTTTTTACGTAAGAACCACCTTAAG | |
| R248S | Sense | GGTCAGTTTCTGGAGTGAATATTTACCACTGA |
| Antisense | CTCCAGAAACTGACCATGAGTGATTTGGATAGA | |
| N366K | Sense | TCATAAGCATGTTGAGGATGGATTCCATGGAGC |
| Antisense | TCAACATGCTTATGAGTCACCTGAACCCCAGTG | |
| Primers used for AADAC genotyping | ||
| V172I | Sense | GGTCAGTTTCTGGAGTGAATATTTACCACTGA |
| Antisense | CTCCAGAAACTGACCATGAGTGATTTGGATAGA | |
| R248S | Sense | CCCACCAGCCTCAGATGAAAATGTCACTGTGAT |
| Antisense | TCTGAGGCTGGTGGGACTTCATCAAAGCTCCCG | |
| Deletion type | ||
| Wild | Sense | CTGGGATGCGGGCAATGTTA |
| Antisense | CTATGACAGAAGCAGGGATTG | |
| Deletion | Sense | GGTACAGCAAGAAGCTCTGATG |
| Antisense | GGTGAATGTCTTCAAGGTTGGAG | |