Table 1

Kinetic parameters for O-desmethyl (S)-naproxen formation by CYP2C9, CYP1A2, and CYP2C8 Supersomes

Reported parameters are the means ± S.D. across three repeated experiments for CYP2C9 and CYP1A2 Supersomes (duplicate experiments for CYP2C8), each with technical duplicates. Comparisons between CYP2C9 and CYP1A2 were assessed using two-tailed unpaired t tests allowing for heteroscedasticity. The asterisks denote statistical significance when compared with the same kinetic parameter in CYP2C9 Supersomes.

P450Km ± S.D.Vmax ± S.D.CLint ± S.D.
μMpmol/min per picomole P450µl/min per picomole P450
CYP2C9280 ± 8.931.7 ± 2.50.11 ± 0.10
CYP1A21000 ± 97*41.7 ± 2.4*0.04 ± 0.003*