Enzyme | Inhibition | CLint | Experimental fm | Corrected fmCYPa | Normalized fmCYPb |
---|---|---|---|---|---|
ml/h per mg protein | % | ||||
No inhibition | 0.223 | ||||
CYP2C8 | Montelukast (2 µM) | 0.145 | 34.9 | 12.8 | 15 |
CYP2C9 | Sulfaphenazole (5 µM) | 0.209 | 6.07 | −1.16 | 0c |
CYP3A | Ketoconazole (1 µM) | 0.0577 | 74.1 | 72.6 | 85 |
aExperimental fmCYP was corrected with inhibitor crossreactivity as described in the Materials and Methods. bCorrected fmCYP was normalized to a total of 100%.
cNegative fmCYP values after correction were considered to be analytical artifacts and set as 0.