Summary of incubation conditions and positive controls used in the P450-Glo assays
| Enzyme | Inhibitor | Dilution Format | Inhibitor Concentration | Incubation Conditions |
|---|---|---|---|---|
| CYP3A4 | Ketoconazole | 16 concentrations/2-fold dilution in duplicates | 57 µM to 1.8 nM | 1 h/RT |
| CYP2C9 | Sulfaphenazole | 57 µM to 1.8 nM | 1 h/ 37°C/ 0.4% BSA | |
| CYP2D6 | Quinidine | 1.4 µM to 0.04 nM | 1 h/RT |