TABLE 3 Cellular accumulation of cannabinoids in MDCKII-P-gp cells
Shown are the AUC ratios of cellular accumulation for 5 μM THC, 0.3 μM 11-OH-THC, and 2.5 μM THC-COOH in MDCKII-P-gp cells in the absence or presence of 5 μM tariquidar. Quinidine (2 μM) was used as a P-gp positive control probe substrate. The AUCs were calculated using the trapezoidal rule with 0, 15, 30, 45, and 60 min as the time points. Data shown are means ± S.D. from at least three independent experiments (n = 4 for THC; n = 5 for 11-OH-THC; and n = 3 for THC-COOH), each conducted in triplicate. Differences in AUC ratio between the absence and presence of tariquidar were analyzed using the paired Student’s t test.