TABLE 1

Kinetic analysis of GSTs against EXE and 17β-DHE

KM, Vmax, and CLINT are expressed as the mean ± S.D. of three independent experiments.

EXE-GS17β-DHE-GS
KMVmaxCLINTKMVmaxCLINT
μMnmol·min−1·mg−1anl·min−1·mg−1aμMnmol·min−1·mg−1anl·min−1·mg−1a
HLC46 ± 120.044 ± 0.00670.0018 ± 0.0001051 ± 140.0020 ± 0.000840.000037 ± 0.0000082
GSTA153 ± 174.7 ± 2.90.085 ± 0.04332 ± 120.48 ± 0.130.017 ± 0.0067
GSTM399 ± 6.014 ± 1.40.14 ± 0.092Not active
GSTM114 ± 4.10.43 ± 0.290.032 ± 0.017Not active
  • aData are expressed as milligrams of total cytosolic protein.