The amounts of unchanged compounds and metabolites after the membrane permeability assays in hiPSC-SIECs and Caco-2 cells.
Unchanged (basal)2.638.2 ± 5.2 Unchanged (basal)49504 ± 31 Unchanged (basal)167450 ± 14 Unchanged (basal)111325 ± 48
| Compounds | Amounts | |
|---|---|---|
| hiPSC-SIECs | Caco-2 cells | |
| pmol/1.5 h | pmol/1.5 h | |
| Raloxifene | ||
| Glucuronide (apical) | 27.0 | 7.3 ± 0.5 |
| Glucuronide (basal) | 68.6 | 7.5 ± 0.4 |
| Daidzein | ||
| Glucuronide (apical) | 238 | 8.3 ± 0.6 |
| Glucuronide (basal) | 202 | 2.8 ± 0.1 |
| Genistein | ||
| Glucuronide (apical) | 221 | 23.7 ± 1.3 |
| Glucuronide (basal) | 158 | 3.5 ± 0.2 |
| Bisphenol A | ||
| Glucuronide (apical) | 65.1 | 16.4 ± 2.0 |
| Glucuronide (basal) | 879 | 26.4 ± 3.5 |
| Sulfate (apical) | 77.0 | 53.7 ± 2.5 |
| Sulfate (basal) | 21.7 | 10.2 ± 1.1 |
The cells were incubated with transport buffer containing each of the food-derived compounds at 37°C for 90 minutes. Unchanged substrates and metabolites were measured using ultraperformance liquid chromatography–tandem mass spectrometry. Data are presented as means for hiPSC-SIECs (n = 2) and means ± S.D. for Caco-2 cells (n = 3).