The total amount of protein in analyte estimated by the label-free quantification methods, averaged (± SD) over 27 samples.

MethodEstimated Total Protein Content
Nominal protein contenta16,667 pmol mg−1
HiN based on BSA8916 ± 1775 pmol mg−1
HiN based on myoglobin34,580 ± 5078 pmol mg−1
iBAQ based on BSA19,265 ± 2937 pmol mg−1
Total Protein Approach (TPA)24,513 ± 4620 pmol mg−1
  • aAmount estimated assuming an average analyte protein molecular mass of 60 kDa; units are pmol mg−1 total protein.