The total amount of protein in analyte estimated by the label-free quantification methods, averaged (± SD) over 27 samples.
| Method | Estimated Total Protein Content |
|---|---|
| Nominal protein contenta | 16,667 pmol mg−1 |
| HiN based on BSA | 8916 ± 1775 pmol mg−1 |
| HiN based on myoglobin | 34,580 ± 5078 pmol mg−1 |
| iBAQ based on BSA | 19,265 ± 2937 pmol mg−1 |
| Total Protein Approach (TPA) | 24,513 ± 4620 pmol mg−1 |
aAmount estimated assuming an average analyte protein molecular mass of 60 kDa; units are pmol mg−1 total protein.