TABLE 2

List of approved PEGylated proteins

NonproprietaryYear of Approval (FDA or EMA)PEG form/size (MW)Conjugation and Linker ChemistryReferences
Pegademase bovine1990Multiple 5 kDaRandom NHS (mPEG succinimidyl
succinate) displacement to lysines.
(Ramos-de-la-Peña and Aguilar, 2019)
Pegaspargase1994Multiple (69-82) linear 5 kDaRandom NHS (mPEG succinimidyl
succinate) displacement to lysines, serines, histidines.
(Belen et al., 2019)
Peginterferon alfa-2b2000Linear 12 kDamPEG succinimidyl
carbonate to histidines
(Ramos-de-la-Peña and Aguilar, 2019)
Peginterferon alfa-2a20012 × 20 kDamPEG succinimidyl
carbonate to lysines
(Ramos-de-la-Peña and Aguilar, 2019)
Pegvisomant2002Multiple linear 5 kDamPEG succinimidyl
succinate displacement to lysines
(Ramos-de-la-Peña and Aguilar, 2019)
Pegfilgrastim200220 kDaN-terminal aldehyde(Ramos-de-la-Peña and Aguilar, 2019)
Pegaptanib20042 × 20 kDamPEG amino(Ramos-de-la-Peña and Aguilar, 2019)
Methoxy PEG epoetin beta2007linear 30 kDamPEG succinimidyl
succinate displacement to lysines
(Ramos-de-la-Peña and Aguilar, 2019)
Certolizumab pegol20082 × linear 20 kDamPEG maleimide to cysteine(Ramos-de-la-Peña and Aguilar, 2019)
Pegloticase2010multiple linear 10 kDamPEG-nitrophenyl
carbonate to lysine
(Ramos-de-la-Peña and Aguilar, 2019)
pegvaliase2010∼9 × 20 kDamPEG succinimidyl
succinate displacement to lysines
(Ramos-de-la-Peña and Aguilar, 2019)
Peginesatide2012Branched 40 kDaLysine branched based (iminodiacetic acid)Eur J Endocrinol. 2017
Peginterferon alfa-1a201420 kDaMethylpropionaldehyde
(mPEG) moiety at the N-terminus
(Ramos-de-la-Peña and Aguilar, 2019)
PEG-rhGH201440 kDaAmino groupsFDA Approval letters 2014
Rurioctocog alfa pegol2016≥1 × 20 kDa branched (2 × 10)Amide bond to lysine(Ramos-de-la-Peña and Aguilar, 2019)
Nonacog beta pegol201740 kDamPEG 2 selective N-linked glycoPEGylation(Ramos-de-la-Peña and Aguilar, 2019)
Elapegademase201880 kDamPEG succinimidyl
carbamate
(Ramos-de-la-Peña and Aguilar, 2019)
Calaspargase pegol201831-39 × 5 kDamPEG succinimidyl carbonate(Ramos-de-la-Peña and Aguilar, 2019)
Damoctocog alfa pegol20182 × 30 kDamPEG maleimide at a site specific cysteineEMA assessment report (2018)
Turoctocog alfa pegol201940 kDaGlycoPEGylation.EMA assessment report (2019)