TABLE 5

Interlaboratory differences between the literatures used for V_max and intrinsic clearance data for k_cat and CL_int,u calculations, including concentrations of UDPGA, alamethicin, MgCl₂, and % of BSA

Literature	UDPGA (mM)^a	Alamethicin^b	MgCl₂ (mM)^b	BSA (%)^c	Buffer^d
(Achour et al., 2018)	5	10μg/ml	5	—	100mM Tris-HCl, pH 7.5
(Al-Jahdari et al., 2006)	3	50μg/ml	8	—	50mM Tris-HCl, pH 7.6
(Benoit-Biancamano et al., 2009)	2	—	10	—	50mM Tris-HCl, pH 7.7
(Bhatt et al., 2019)	2.5	100μg/ml	—	0.01	100mM phosphate, pH 7.4
(Chen et al., 2018)	—	25mg/ml	5	—	50mM Tris-HCl, pH 7.4
(Cubitt et al., 2009)	5	50μg/ml	10	—	100mM phosphate, pH 7.1
(Gill et al., 2012)	5	50μg/mg protein	3.45	1 to 2^e	100mM phosphate, pH 7.1
(Gill et al., 2013)	5	50μg/ml	3.45	—	100mM phosphate, pH 7.1
(Knights et al., 2016)	5	50μg/mg protein	4	—	100mM phosphate, pH 7.4
(Komura and Iwaki, 2011)	2	50μg/mg protein	10	—	100mM Tris-HCl, pH 7.4
(Liang et al., 2011)	5	25μg/ml	5	—	50mM Tris-HCl, pH 7.4
(Miles et al., 2005)	3	50μg/mg protein	10	—	75mM Tris-HCl, pH 7.45
(Picard et al., 2005)	2	—	10	—	100mM Tris-HCl, pH 7.4
(Rowland et al., 2008)	5	50μg/mg protein	4	—	100mM phosphate, pH 7.4
(Scotcher et al., 2017)	5	50μg/mg protein	3.45	1	100mM phosphate, pH 7.4
(Shimizu et al., 2007)	3	50μg/ml	10	—	50mM Tris-HCl, pH 7.4
(Soars et al., 2001)	5	—	10	—	100mM Tris-maleate, pH 7.4
(Soars et al., 2003)	5	50μg/ml	1	—	100mM phosphate, pH 7.1
(Walsky et al., 2012)	5	10μg/ml	5	—	100mM Tris-HCl, pH 7.5

^aMinimum 5mM required for reliable glucuronidation activities for k_cat and CL_int,u calculations (Miners et al., 2021).
^bFactors influencing measured V_max specifically (Miners et al., 2021).
^cReduces substrate Km, impacting on measured CL_int,u (Miners et al., 2021).
^dIncreases glucuronidation activities, reflected as greater mean CL_int,u (Badée et al., 2019).
^eUGT1A1, 2% BSA; UGT1A3, 1% BSA.
—, No data.