Protein | Peptide Sequence | IS | RT | Q1 [M + 2H]2+ | Q3 [M + H]+ |
---|---|---|---|---|---|
min | Da | Da | |||
P-gp | IATEAIENFR | BCRP-H | 15.8 | 582.3 | 979.5 |
BCRP | ENLQFSAALR | BCRP-H | 16.5 | 574.8 | 792.4 |
MRP2 | GINLSGGQK | MRP2-H | 9.8 | 437.2 | 703.4 |
MRP3 | QGELQLLR | MRP2-H | 10.0 | 366.2 | 501.3 |
MRP4 | APVLFFDR | MRP4-H | 19.9 | 482.8 | 697.4 |
OAT1 | TSLAVLGK | OAT1-H | 14.0 | 394.7 | 600.4 |
OAT2 | VGGFGPFQLR | MRP4-H | 20.4 | 539.3 | 864.5 |
OAT3 | DITSAK | MRP2-H | 4.8 | 317.7 | 406.2 |
OAT5 | ILSQDDLLR | BCRP-H | 16.1 | 536.8 | 846.4 |
OATP4C1 | DFPTAVK | OAT1-H | 12.2 | 389.2 | 515.3 |
MRP2-H | GINL*SGGQK | — | 9.8 | 440.7 | 710.4 |
MRP4-H | TSLA*VLGK | — | 20.4 | 486.3 | 704.4 |
BCRP-H | ENLQFSA*ALR | — | 16.5 | 576.9 | 668.4 |
OAT1-H | TSLA*VLGK | — | 14.0 | 396.7 | 491.3 |
Multiple reaction monitoring transitions used for detection are listed as doubly charged parent ion (Q1) and singly charged fragment ion (Q3) used for quantification.
* Indicates 13C/16N heavy isotope labeled amino acid internal standards (IS). RT indicates retention time for liquid chromatography method. — Indicates that no IS was used.