Table 1

Primer sequences used for the construction of expression plasmids The nucleotide numbering refers to the transcription start site as 1.

IsoformSequence’(5′ → 3′)Product Size (bp)RegionRef. Seq
AKR1C1 SGAAAGAAACATTTGCCAGCC1144149–1292NM_001353
 ASTGCTGTAGCTTGCTGAAATCA
AKR1C2 SCCACAGGTAAGAAACGGTTGA1294229–1522NM_001354
 ASAGCTGTAGCTTACTGAAGTCG
AKR1C3 SCAGACAAGTGACAGGGAATGGA101953–1071NM_003739
 ASTCTGGTAGACATCAGGCAAAGC
AKR1C4 SAGTGGCAAGCAATGGATC101523–1037NM_001818
 ASCTTCTGCTAGATGTCGTGC
CBR1 STTCTCCACGCAGGTGTTC1078157–1234NM_001757
 ASGCATCAGAGGAAATCACAAAAG
HSD11B1 SGTCCTACAGGAGTCTTCAGGC0928120–1047NM_005525
 ASCCCTCAGGGAGTTCCTACTTG