Regular ArticleEffects of Freezing, Thawing, and Storing Human Liver Microsomes on Cytochrome P450 Activity
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UPLC-MS/MS analysis of the Michaelis-Menten kinetics of CYP3A-mediated midazolam 1′- and 4-hydroxylation in rat brain microsomes
2021, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesCitation Excerpt :The heart was then perfused with a cold saline solution at a flow rate of 25 mL/min for 6 min to remove the blood from the brain and other organs. Perfusion of tissue removes contamination of microsomes by hemoglobin in the residual blood, which causes a uniform decrease in the specific activities of CYP enzymes [27]. The whole brain was then removed, snap-frozen in liquid nitrogen, and stored at –80 °C.
A tutorial for the assessment of the stability of organometallic complexes in biological media
2020, Journal of Organometallic ChemistryCitation Excerpt :Whereas freshly extracted microsomes are often employed in (pre)clinical studies (eg. from cancer patients), microsome solutions can also be readily acquired from various suppliers. The enzymatic activity of the microsomes can be maintained with correct storage at −80 °C between freeze-thaw cycles [21], and thus the same batch of microsomes can easily be used for different experiments or compounds without the additional deviation between microsome batches [22]. The microsomes can either come from a single donor, or, as it is preferred because it gives a better median and less deviations between the experiments, from a pool of different donors, usually around 50.
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Present address: Phoenix International, Life Sciences Incorporated, St. Laurent (Montreal), Quebec, H4R 2N6 Canada.
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To whom correspondence should be addressed at Department of Pharmacology, Toxicology, and Therapeutics, University of Kansas Medical Center, Kansas City, KS 66160-7417. Fax: (913) 588-7330.