Regular ArticleFluorescence-Based Assay for the Interaction of Small Molecules with the Human Renal Organic Anion Transporter 1
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2021, Analytical BiochemistryCitation Excerpt :Therefore, the development of non-radioactive screening methods for URAT1 inhibitors is highly desirable. Cihlar et al. revealed that fluorescein and its analogs are frequently used fluorescent substrates, which can be transported by organic anion transporter 1 (OAT1, SLC22A6) [20]. Since OAT1 and URAT1 belong to the same OATs (SLC22 family), we designed and obtained human embryonic kidney 293T cells that stably express human URAT1 (hURAT1), and chose fluorescein and its analogs as potential substrates to develop a non-radioactive cell-based screening system for URAT1 inhibitors.
Organic anion transporter 1 (OAT1/SLC22A6) enhances bioluminescence based on D-luciferin–luciferase reaction in living cells by facilitating the intracellular accumulation of D-luciferin
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