Biochemical and Biophysical Research Communications
Regular ArticleExpression and Functional Characterization of the Mammalian Intestinal Peptide Transporter PepT1 in the Methylotropic YeastPichia pastoris☆,☆☆
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This work was supported by the Deutsche Forschungsgemeinschaft, Projekt B12, SFB 249 to H. Daniel.
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Abbreviations: PepT1, rabbit intestinal peptide transporterAOX1, alcohol oxidase1; MutS, slow methanol utilization; Mut+, same methanol utilization pathway as wild type; ACE, angiotensin converting enzyme; OD, optical density 600nm (one OD equals approximately 5×107cells);
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Corresponding author. Institute of Nutritional Sciences, Justus-Liebig-University Giessen, Wilhelmstrasse 20, D-35392 Giessen, Germany. Fax: Germany 641 99 39049. E-mail: hannelore.daniel@ ernaehrung.uni-giessen.de.