Regular Article
Genomic Organization and Tissue-Specific Expression of Splice Variants of Mouse Organic Anion Transporting Polypeptide 2☆,☆☆

https://doi.org/10.1006/bbrc.2001.4387Get rights and content

Abstract

cDNAs that code for mouse organic anion transporting polypeptide 2 (oatp2) have been cloned. At least three forms of mouse oatp2 cDNAs containing the same coding sequence were isolated. The common coding sequence is for a protein of 670 amino acids with 12 putative transmembrane domains. The deduced amino acid sequence of the mouse oatp2 shares 89% identity with the reported rat oatp2. Cloning and analysis of mouse oatp2 gene indicates that these isoforms are alternatively spliced products from the same gene. Heterogeneity was observed in the 5′-untranslated region of the cDNAs. Two of the three isoforms lacked the noncoding exon 3 sequence. Northern-blot hybridization analysis using the exon 3-specific probes demonstrated that mouse oatp2 mRNA containing exon 3 sequence is expressed in heart and lung, whereas exon 1-, 2-, and 17-specific probes detected mRNA only in brain and liver. The mouse oatp2 gene consists of 17 exons, including three noncoding exons, and 16 introns. All of the introns are flanked by GT-AG splice sequences except for intron 10 that is flanked by GC-AG splice sequence.

References (36)

  • T.A. Kupriyanova et al.

    Akt-2 binds to Glut4-containing vesicles and phosphorylates their component proteins in response to insulin

    J. Biol. Chem.

    (1999)
  • J. König et al.

    Localization and genomic organization of a new hepatocellular organic anion transporting polypeptide

    J. Biol. Chem.

    (2000)
  • R. Lu et al.

    Molecular cloning of the gene for the human prostaglandin transporter hPGT: gene organization, promoter activity, and chromosomal localization

    Biochem. Biophys. Res. Commun.

    (1998)
  • I. Tooyama et al.

    A protein encoded by an alternative splice variant of choline acetyltransferase mRNA is localized preferentially in peripheral nerve cells and fibers

    J. Chem. Neuroanat.

    (2000)
  • V. Boonyaratanakornkit et al.

    Progesterone stimulation of human insulin-like growth factor-binding protein-5 gene transcription in human osteoblasts is medicated by a CACCC sequence in the proximal promoter

    J. Biol. Chem.

    (1999)
  • K.A. O'Leary et al.

    Molecular basis for cell-specific regulation of the NADPH-cytochrome P450 oxidoreductase gene

    Arc. Biochem. Biophys.

    (2000)
  • M. Müller et al.

    The secretory function of the liver: New aspects of hepatobiliary transport

    J. Hepatol.

    (1998)

    • Cited by (0)

    ☆

    Sequence data from this article have been deposited in DDBJ/EMBL/GenBank data bases under the Accession Nos. AB031814 and AB043009 through AB043023.

    ☆☆

    This work was supported by NIEMS Grant ES-09649 and ES-03192.

    1

    Present address: Department of Drug Metabolism and Molecular Toxicology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.

    2

    To whom correspondence should be addressed at Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160-7417. Fax: (913) 588-7501. E-mail: [email protected].

    View full text
    Copyright © 2001 Academic Press. All rights reserved.