Regular ArticleValidation of Precision-Cut Liver Slices in Dynamic Organ Culture as anin VitroModel for Studying CYP1A1 and CYP1A2 Induction☆,☆☆
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Polymorphic cytochromes P450 in non-human primates
2022, Advances in PharmacologyCitation Excerpt :A similar slight induction has been noted for cynomolgus CYP1A2 (Edwards et al., 1994). Similarly, CYP1A1 mRNA and protein levels increased in human liver slices treated with TCDD, resulting in increased enzyme activity; in contrast, the induction of CYP1A2 mRNA was modest (Drahushuk et al., 1996). In marmosets and cynomolgus macaques, CYP1A2 mRNA is induced by 3-methylcholanthrene (Sakuma et al., 1997, 1998), similar to human CYP1A2 in primary hepatocytes (Rhodes, Otten, Hingorani, Hartley, & Franklin, 2011).
The marmoset cytochrome P450 superfamily: Sequence/phylogenetic analyses, genomic structure, and catalytic function
2020, Biochemical PharmacologyCitation Excerpt :Similarly, the treatment of human liver slices with TCDD in dynamic organ culture increases the levels of P450 1A1 mRNA and the protein and, consequently, results in increased enzyme activity. At the same time, the induction of P450 1A2 mRNA in liver slices was more modest [26]. In marmosets and cynomolgus macaques, P450 1A2 mRNA is commonly induced by 3-methylcholanthrene [21,23]; similar results were obtained for P450 1A2 in primary human hepatocytes [27].
Tissue distribution of dioxin-like compounds: Potential impacts on systemic relative potency estimates
2013, Toxicology LettersCitation Excerpt :In this study, the EC50 for hepatic CYP1A1 activity for TCDD was approximately 5 ng/g liver. Other studies in rats and mice, including SYSTEQ data, have reported EC50 values in the same range for induction of hepatic CYP1A1 by TCDD, irrespective of the administration protocol (DeVito et al., 1997; Diliberto et al., 2001; Drahushuk et al., 1996; van Birgelen et al., 1995a; Vanden Heuvel et al., 1994). Thus, regardless of the use of a single or multiple oral dosages, it is shown that EC50 values for induction of hepatic CYP1A1 by TCDD are comparable if based on tissue concentrations.
Fused mesoionic heterocyclic compounds are a new class of aryl hydrocarbon: Receptor (ahr) agonist of exceptional potency
2012, ToxicologyCitation Excerpt :Samples were stored at −20 °C. Activation of the AhR was estimated through measurement of the induction of CYP1A1 mRNA (Drahushuk et al., 1996; Gaylor and Aylward, 2004; Vanden Heuvel et al., 1994) in rat H4IIE cells and human MCF-7 cells using quantitative real-time PCR (qRT-PCR). Further analysis of CYP1B1 and CYP1A2 was conducted in rat H4IIE cells.
Gestational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin alters retinoid homeostasis in maternal and perinatal tissues of the Holtzman rat
2007, Toxicology and Applied Pharmacology
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Supported by NIEHS Grant R01 ES06556-01.
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Presented in part at the Annual Meeting of the Society of Toxicology (Toxicologist15,63, 1995).