Abstract
Mass spectrometry (MS)-based quantitation of plasma proteomes is challenging due to the extremely wide dynamic range and molecular heterogeneity of plasma samples. However, recent advances in technology, MS instrumentation, and bioinformatics have enabled in-depth quantitative analyses of very complex proteomes, including plasma. Specifically, recent improvements in both label-based and label-free quantitation strategies have allowed highly accurate quantitative comparisons of expansive proteome datasets. Here we present a method for in-depth label-free analysis of human plasma samples using MaxQuant.
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Acknowledgments
This work was supported by NIH Grants RO1HD076279, RO1CA131582, and WW Smith Charitable Trust Grants H1205 and H1305 (D.W. Speicher), PA Department of Health Commonwealth Universal Research Enhancement (CURE) Program Grant (B. Ky), as well as CA10815 (NCI core grant to the Wistar Institute).
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Beer, L.A., Liu, P., Ky, B., Barnhart, K.T., Speicher, D.W. (2017). Efficient Quantitative Comparisons of Plasma Proteomes Using Label-Free Analysis with MaxQuant. In: Greening, D., Simpson, R. (eds) Serum/Plasma Proteomics. Methods in Molecular Biology, vol 1619. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7057-5_23
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DOI: https://doi.org/10.1007/978-1-4939-7057-5_23
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