Abstract
The concentrations of glucuronidated and sulfated bile acids in the serum of 15 patients with acute hepatitis were determined by mass fragmentography. Total serum bile acid levels were 13.79–444.10 μmol/liter, and the percentages of glucuronidated and sulfated bile acids were in the wide ranges of 1.7–33% and 2.4–49%, respectively. In four of the five patients for whom serum bile acids were analyzed serially from the acute to the recovery stages of acute hepatitis, the decrease of the glucuronidated and sulfated bile acids was slower than that of nonglucuronidated, nonsulfated bile acids. Thus, the relative proportion of the glucuronides and sulfates in total bile acids apparently increased during the recovery phase. The mechanism for the relative predominance of bile acid esters in serum during recovery is unknown but might reflect an improved excretion of the nonesterified bile acids into bile after the rapid recovery of intrahepatic cholestasis.
Similar content being viewed by others
References
Rudman D, Kedall FE: Bile acid content of human serum. 1. serum bile acids in patients with hepatic disease. J Clin Invest 36:530–537, 1957
Frosch B, Wagener H: Quantitative determination of conjugated bile acids in serum in acute hepatitis. Nature 213: 404–405, 1967
Makino I, Nakagawa N, Mashimo K: Conjugated and unconjugated serum bile acid levels in patients with hepatobiliary diseases. Gastroenterology 56:1033–1039, 1969
Carey Jr JB: Bile salt metabolism in man.In The Bile Acids, Chemistry, Physiology, and Metabolism, Vol. 2. PP Nair, D Kritchevsky (eds). New York, Plenum, 1973, pp 55–82
Makino I, Hashimoto H, Shinozaki K, Yoshino K, Nakagawa S: Sulfated and nonsulfated bile acids in urine, serum, and bile of patients with hepatobiliary diseases. Gastroenterology 68:545–553, 1975
Campbell CB, McGuffie C, Powell LW: The measurement of sulphated and nonsulfated bile acids using gas-liquid chromatography. Clin Chim Acta 63:249–262, 1975
Campbell CB, McGuffie C, Powell LW, Roberts RK, Stewart AW: Postprandial changes in serum concentrations of individual bile salts in normal subjects and patients with acute hepatitis. Am J Dig Dis 23:599–608, 1978
Back P, Spaczynski K, Gerok W: Bile-salt glucuronides in urine. Hoppe-Seyler's Z Physiol Chem 355:749–752, 1974
Back P: Bile acid glucuronides. Hoppe-Seyler's Z Physiol Chem 357:213–217, 1976
Fröhling W, Stiehl A: Bile salt glucuronides: Identification and quantitative analysis in the urine of patients with cholestasis. Eur J Clin Invest 6:67–74, 1976
Stiehl A, Becker M, Czygan P, Fröhling W, Kommerell B, Rotthauwe HW: Bile acids and their sulfated and glucuronidated derivatives in bile, plasma, and urine of children with intrahepatic cholestasis: Effects of phenobarbital treatment. Eur J Clin Invest 10:307–316, 1978
Almé B, Norden Å, Sjövall J: Glucuronides of unconjugated 6-hydroxylated bile acids in urine of patients with malabsorption. Clin Chim Acta 86:251–259, 1978
Almé B, Sjövall J: Analysis of bile acid glucuronides in urine: Identification of 3α, 6α, 12α-trihydroxy-5β-cholanoic acid. J Steroid Biochem 13:907–916, 1980
Stiehl A, Raedsch G, Rudolph G, Czygan P, Walker S: Analysis of bile acid glucuronides in urine: Group separation on a lipophilic anion exchanger. Clin Chim Acta 123:275–285, 1982
Takikawa H, Otsuka H, Beppu T, Seyama Y, Yamakawa T: Quantitative determination of bile acid glucuronides in serum by mass fragmentography. J Biochem 92:985–998, 1982
Parmentier G, Eyssen H: Synthesis of the specific monosulfates of cholic acid. Steroids 26:721–729, 1975
Beppu T, Seyama Y, Kasama K, Yamakawa T: Quantitative determination of individual nonsulfated bile acids and sulfated lithocholic acid in serum by mass fragmentography. J Biochem 89:167–175, 1981
Takikawa H, Beppu T, Seyama Y, Wada T: Quantitative determinations of nonsulfated bile acids in the serum of patients with hepatobiliary diseases by mass fragmentography. Gastroentrol Jpn 18:246–251, 1983
Takikawa H, Otsuka H, Beppu T, Seyama Y, Yamakawa T: Serum concentrations of bile acid glucuronides in hepatobiliary diseases. Digestion 27:189–195, 1983
Takikawa T, Beppu T, Seyama Y: Profiles of bile acids and their glucuronide and sulphate conjugates in the serum, urine and bile of patients undergoing bile drainage. Gut 26:38–42, 1985
Takikawa H, Beppu T, Seyama Y: Urinary concentrations of bile acid glucuronides and sulfates in hepatobiliary diseases. Gastroenterol Jpn 19:104–109, 1984
Hofmann AF, Korman MG, Krugman S: Sensitivity of serum bile acid assay for detection of liver damage in viral hepatitis type B. Am J Dig Dis 19:908–910, 1974
Mishler IV JM, Barbosa L, Mihalko LJ, McCarter H: Serum bile acids and alanine aminotransferase concentrations. JAMA 246:2340–2344, 1981
Author information
Authors and Affiliations
Additional information
This study was supported by a Grant-in-Aid for Scientific Research (A) from the Ministry of Education, Science and Culture of Japan (No. 57440062).
Rights and permissions
About this article
Cite this article
Takikawa, H., Beppu, T., Seyama, Y. et al. Glucuronidated and sulfated bile acids in serum of patients with acute hepatitis. Digest Dis Sci 31, 487–491 (1986). https://doi.org/10.1007/BF01320312
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01320312