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Separation ofCimicifuga racemosa triterpene glycosides by reversed phase high performance liquid chromatography and evaporative light scattering detection

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Summary

The main triterpene glycosides ofCimicifuga racemosa were separated by reversed phase HPLC, using a C-18 column, Evaporative Light Scattering (ELS) detection and a grient system consisting of water, acetonitrile and reagent alcohol. Within 35 min three main glycosides could be separated and quantified in the methanolic root extract with detection limits of 10.5, 15.6 and 31.6 μg·mL−1 respectively. The method was successfully used, to analyzed differentCimicifuga racemosa market products, as well as to distinguish between otherCimicifuga samples from China.

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References

  1. Lloyd, L.U.; Lloyd, C.G. Drugs and Medicines of North America Vol. 1 Ranunculaceae, L.U. & C.G. Loyd, Cincinati, 1884.

    Google Scholar 

  2. Mooney, J.; Olbrechts, F.M. The Swimmer Manuscript: Cherokee, Sacred Formulas and Medicinal Prescriptions, Smithonian Institution Bureau of American Ethnology, Washington, 1932.

    Google Scholar 

  3. Zheng, Q.Y.; He, K.; Pilkington, L.; Yu, S.; Zheng, B. in Phytochemicals, and Phytopharmaceuticals: Shahidi, F.; Ho, C.T., Eds, AOCS Press, Champaign 2000.

    Google Scholar 

  4. Kruse, S.; Lohning, A., Pauli, G.; Winterhoff, H.; Nahrstedt, A.Planta Med 1999,65, 763–764.

    CAS  Google Scholar 

  5. Düker, E.; Kopansky, H.; Jarry, H.; Wuttke, W.Planta Med 1991,57, 420–424.

    Google Scholar 

  6. Jarry, H.; Harnischfeger, G., Düker, E.Planta Med 1985,51, 316–319.

    Google Scholar 

  7. Hirabayashi, T.; Ochiai, H.; Sakai, S.; Nakajima, K.; Terasawa, K.Planta Med 1995,61, 221–226.

    Article  CAS  Google Scholar 

  8. Liske, E.; Wüstenberg, P.Menopause 1998,5, 250.

    Google Scholar 

  9. Bedir, E.; Khan, I.A.Chem. Pharm. Bull 2000,48, 425–427.

    CAS  Google Scholar 

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Authors and Affiliations

  1. National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, 38677, University, MS, USA

    M. Ganzera, E. Bedir & I. A. Khan

  2. Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, 38677, University, MS, USA

    E. Bedir

  3. Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey

    I. A. Khan

Authors
  1. M. Ganzera
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  2. E. Bedir
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  3. I. A. Khan
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Ganzera, M., Bedir, E. & Khan, I.A. Separation ofCimicifuga racemosa triterpene glycosides by reversed phase high performance liquid chromatography and evaporative light scattering detection. Chromatographia 52, 301–304 (2000). https://doi.org/10.1007/BF02491021

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  • DOI: https://doi.org/10.1007/BF02491021

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