Abstract.
Covalent binding of various clinically important nitrogen mustards to the cysteine-34 residue of human serum albumin, in vitro and in vivo, is demonstrated. A rapid method for detection of these adducts is presented, based on liquid chromatography-tandem mass spectrometry analysis of the adducted tripeptide Cys*-Pro-Phe after digestion of the protein with Pronase.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Electronic Publication
Rights and permissions
About this article
Cite this article
Noort, D., Hulst, A.G. & Jansen, R. Covalent binding of nitrogen mustards to the cysteine-34 residue in human serum albumin. Arch Toxicol 76, 83–88 (2002). https://doi.org/10.1007/s00204-001-0318-2
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s00204-001-0318-2