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Covalent binding of nitrogen mustards to the cysteine-34 residue in human serum albumin

  • Toxicokinetics and Metabolism
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Abstract.

Covalent binding of various clinically important nitrogen mustards to the cysteine-34 residue of human serum albumin, in vitro and in vivo, is demonstrated. A rapid method for detection of these adducts is presented, based on liquid chromatography-tandem mass spectrometry analysis of the adducted tripeptide Cys*-Pro-Phe after digestion of the protein with Pronase.

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Noort, D., Hulst, A.G. & Jansen, R. Covalent binding of nitrogen mustards to the cysteine-34 residue in human serum albumin. Arch Toxicol 76, 83–88 (2002). https://doi.org/10.1007/s00204-001-0318-2

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  • DOI: https://doi.org/10.1007/s00204-001-0318-2

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