Abstract
Mass cytometry addresses the analytical challenges of polychromatic flow cytometry by using metal atoms as tags rather than fluorophores and atomic mass spectrometry as the detector rather than photon optics. The many available enriched stable isotopes of the transition elements can provide up to 100 distinguishable reporting tags, which can be measured simultaneously because of the essential independence of detection provided by the mass spectrometer. We discuss the adaptation of traditional inductively coupled plasma mass spectrometry to cytometry applications. We focus on the generation of cytometry-compatible data and on approaches to unsupervised multivariate clustering analysis. Finally, we provide a high-level review of some recent benchmark reports that highlight the potential for massively multi-parameter mass cytometry.
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Acknowledgments
The University of Toronto investigators gratefully acknowledge receipt of financial support from the Ontario government through the Ontario Research Fund—Global Leadership in Genomics and Life Sciences (ORF-GL2-01-003). Scott Tanner further wishes to acknowledge previous enabling research funding from Genome Canada (Applied Human Health, and Technology Development) and ongoing support from the NIH-Office of AIDS Research.
Conflict of interest
The authors are employees of, and receive remuneration from, DVS Sciences Inc. Scott Tanner, Vladimir Baranov, Olga Ornatsky, and Dmitry Bandura are co-founders of and equity shareholders in DVS Sciences Inc. Scott Tanner is a member of the Board of Directors of DVS Sciences, Inc.
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This paper is a Focussed Research Review based on a presentation given at the Tenth Annual Meeting of the Association for Cancer Immunotherapy (CIMT), held in Mainz, Germany, May 23–25, 2012. It is part of a CII series of Focussed Research Reviews and meeting report.
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Tanner, S.D., Baranov, V.I., Ornatsky, O.I. et al. An introduction to mass cytometry: fundamentals and applications. Cancer Immunol Immunother 62, 955–965 (2013). https://doi.org/10.1007/s00262-013-1416-8
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DOI: https://doi.org/10.1007/s00262-013-1416-8