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8-Hydroxycannabinol: a new metabolite of cannabinol formed by human hepatic microsomes

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Abstract

Metabolism of cannabinol (CBN) was studied in vitro using hepatic microsomes from human livers. The metabolites formed were analyzed by thinlayer chromatography (TLC) and identified by gas chromatography-mass spectrometry as their trimethylsilyl derivatives. 11-Hydroxy-CBN, the major metabolite, was detected together with a smaller amount of another mono-hydroxylated metabolite. The minor metabolite was identified as 8-hydroxy-CBN, after comparing its Rf value by TLC, retention time by GC, and the mass spectrum with those of the authentic compound. 8-Hydroxy-CBN was confirmed to be a new metabolite of CBN formed by human hepatic microsomes.

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Authors and Affiliations

  1. Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Ho-3 Kanagawa-machi, Kanazawa, 920-1181, Japan

    Kazuhito Watanabe, Satoshi Yamaori, Tatsuya Funahashi & Toshiyuki Kimura

  2. Department of Hygienic Chemistry, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, Nobeoka, 882-8508, Japan

    Ikuo Yamamoto

Authors
  1. Kazuhito Watanabe
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  2. Satoshi Yamaori
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  3. Tatsuya Funahashi
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  4. Toshiyuki Kimura
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  5. Ikuo Yamamoto
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Correspondence to Kazuhito Watanabe.

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Watanabe, K., Yamaori, S., Funahashi, T. et al. 8-Hydroxycannabinol: a new metabolite of cannabinol formed by human hepatic microsomes. Forensic Toxicol 24, 80–82 (2006). https://doi.org/10.1007/s11419-006-0016-0

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  • DOI: https://doi.org/10.1007/s11419-006-0016-0

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