Studies on pig liver microsomes. I. Enzymic and pigment composition of different microsomal fractions

doi:10.1016/0003-9861(58)90095-X

Archives of Biochemistry and Biophysics

Volume 77, Issue 2, October 1958, Pages 493-509

https://doi.org/10.1016/0003-9861(58)90095-X Get rights and content

Abstract

1.
1. A possible seasonal effect in pig liver microsomes is described.
2.
2. A crude fractionation of microsomes into light and bulk fractions is described. Examination in the electron microscope indicates that these contain predominantly smooth-surfaced and rough-surfaced microsomes, respectively.
3.
3. Chemical analysis and observation of the behavior of the microsomes on digestion and solubilization indicate that the smooth- and rough-surfaced microsomes are biochemically distinct, with the smooth-surfaced ones being rich in cytochrome b₅ whereas the rough-surfaced ones are rich in noncytochrome heme and contain a tan pigment.
4.
4. Properties of some microsomal components, including a CO-binding pigment and a soluble yellow pigment, have been described.
5.
5. The binding of hemoglobin to microsomes is described.
6.
6. The effects of low pH and solubilizing agents on microsomes are described.
7.
7. The properties of a microsomal substance (designated “acceptor”) which is reduced by cytochrome b₅ are described, and its origins and importance are discussed.

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^☆: A preliminary report of part of this work was presented at the meeting of the Biophysical Society, Cambridge, Mass., Feb. 5, 1958.

²: Public Health Service Research Fellow of the National Cancer Institute.

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Studies on pig liver microsomes. I. Enzymic and pigment composition of different microsomal fractions☆

Abstract

J. Biol. Chem

Arch. Biochem. Biophys

J. Biol. Chem

Arch. Biochem. Biophys

Biochim. et Biophys. Acta

J. Biol. Chem

J. Biol. Chem

J. Biol. Chem

J. Biol. Chem

J. Biol. Chem

Biochim. et Biophys. Acta

Arch. Biochem. Biophys

Biochim. et Biophys. Acta