Preparation of monospecific antibodies against two forms of rat liver cytochrome P-450 and quantitation of these antigens in microsomes☆
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Cited by (142)
Cytochrome P450 Enzymes
2018, Comprehensive Toxicology: Third EditionHuman mitochondrial cytochrome P450 27C1 is localized in skin & preferentially desaturates trans-retinol to 3,4-dehydroretinol
2017, Journal of Biological ChemistryCitation Excerpt :The sera were compared, and the best serum was used based on specificity and titer. Rabbit anti-27C1 antibody was purified by affinity chromatography with purified recombinant protein (84). Purified 27C1 was coupled to CNBr-activated Sepharose (GE Healthcare) according to the manufacturer's instructions.
Cytochrome P450 Enzymes
2010, Comprehensive Toxicology, Second EditionAryl hydrocarbon receptor response to indigoids in vitro and in vivo
2004, Archives of Biochemistry and BiophysicsCitation Excerpt :Protein concentrations were estimated using a bicinchroninic acid (BCA) method according to manufacturer’s recommendations (Pierce, Rockford, IL). SDS–polyacrylamide gel electrophoresis/immunoblotting (“Western blotting”) was done as described elsewhere [36,37] using an immunoglobulin G fraction (0.1 mg protein/ml) of rabbit antiserum raised against purified rat P450 1A1 [38] and cross-absorbed against rat liver microsomes prepared from untreated rats coupled to Sepharose to remove antibodies reacting with other P450s [39]. Blots were developed with 4-chloro-1-naphthol and imaging was done using a flatbed scanner and the program ImageQuant (Molecular Dynamics, Sunnyvale, CA; now available from Amersham Biosciences, Piscataway, NJ), with the same size of rectangle used for every band.
Metabolite complex formation of orphenadrine with cytochrome P450: Involvement of CYP2C11 and CYP3a isozymes
1996, Biochemical Pharmacology
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A preliminary report of this work has been published in abstract form, Thomas, P. E., Korzeniowski, D., Ryan, D., and Levin, W. (1978) Fed. Proc.37, 766.