Elsevier

Biochemical Pharmacology

Volume 28, Issue 11, 1 June 1979, Pages 1777-1781
Biochemical Pharmacology

Inhibition of mono-oxygenase activities by 1,1,1-trichloropropene 2,3-oxide, an inhibitor of epoxide hydrase, in rat liver microsomes

https://doi.org/10.1016/0006-2952(79)90540-9Get rights and content

Abstract

Addition of 1,1,1-trichloropropene 2,3-oxide (TCPO), an inhibitor of microsomal epoxide hydrase, to rat liver microsomes caused a type I spectral change, and its magnitude was increased by pretreatment of animals with phenobarbital (PB) but not with 3-methylcholanthrene and polychlorinated biphenyls. TCPO inhibited aminopyrine N-demethylation competitively and prevented covalent binding of 2,4,2',4'-tetrachlorobiphenyl to macromolecules catalyzed by liver microsomes, although it stimulated benzolalpyrene hydroxylation significantly. It is suggested that TCPO interacts with cytochrome P-450, especially a species of the cytochrome which is inducible by PB administration, and thus inhibits mono-oxygenase activities of liver microsomes.

References (32)

  • P. Sims et al.
  • H. Kappus et al.

    Biochem. Pharmac.

    (1975)
  • J.C. Leutz et al.

    Archs. Biochem. Biophys.

    (1975)
  • C.S. Yang et al.

    Biochem. Pharmac.

    (1975)
  • T. Omura et al.

    J. biol. Chem.

    (1964)
  • O.H. Lowry et al.

    J. biol. Chem.

    (1951)
  • D.W. Nebert et al.

    J. biol. Chem.

    (1968)
  • Y. Imai et al.

    Biochem. biophys. Res. Commun.

    (1966)
  • H. Vainio

    Chem. Biol. Interact.

    (1974)
  • C. Hashimoto et al.

    Biochem. biophys. Res. Commun.

    (1976)
  • D.A. Haugen et al.

    J. biol. Chem.

    (1975)
  • G. Sundström et al.

    Chemosphere

    (1976)
  • H. Mukhtar et al.

    Biochem. Pharmac.

    (1976)
  • J.R. Gillette et al.

    A. Rev. Pharmac.

    (1974)
  • C. Heiderberger

    A. Rev. Biochem.

    (1975)
  • T. Shimada

    Bull. evir. Contain. Toxic.

    (1976)
  • Cited by (0)

    View full text