Research paperThe isoenzyme pattern of cytochrome P450 in rat hepatocytes in primary culture, comparing different enzyme activities in microsomal incubations and in intact monolayers
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2005, Mutation Research - Genetic Toxicology and Environmental MutagenesisCitation Excerpt :The S9-mix consisted of rat-liver microsome preparations (S9-fraction) obtained from Aroclor-1254-treated male Wistar rats and NADPH-generating co-factors using the standard procedures [31,32]. The total protein content and the activity of the cytochrome P450 isoenzyme P450-1A were determined to be 29.15 mg/ml (Lowry method) [33] and 51.58 pmol/ml/min/mg protein (ethoxyresorufin O-deethylase (EROD) method) [34], respectively. Compounds 1–8 were dissolved in DMSO (500 μg/ml) and tested at five different concentrations (1–4; 0.0, 1.0, 2.0, 4.0, 8.0, 12.0 and 20.0 μg/plate and 5–8; 0.0, 1.0, 5.0, 15.0 and 20.0 μg/plate).
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