Rat adult hepatocytes in primary pure and mixed monolayer culture: Comparison of the maintenance of mixed function oxidase and conjugation pathways of drug metabolism
References (37)
- et al.
Reliability of the hepatocyte primary culture/DNA repair test in testing of coded carcinogens and noncarcinogens
Mutat Res
(1982) - et al.
The cytotoxicity of halothane in isolated hepatocytes: evidence for two different mechanisms
Toxic In Vitro
(1988) - et al.
Human adult hepatocytes in primary monolayer culture. Maintenance of mixed function oxidase and conjugation pathways of drug metabolism
Biochem Pharmacol
(1987) - et al.
Regulation of heme metabolism and cytochrome P-450 levels in primary culture of rat hepatocytes in a defined medium
Biochem Pharmacol
(1984) - et al.
Studies on the maintenance of cytochromes P-450 and b5, monooxygenases and cytochrome reductases in primary cultures of rat hepatocytes
FEBS Lett
(1985) - et al.
Long-term maintenance of hepatocytes in primary culture in the presence of DMSO: further characterization and effect of nafenopin, a peroxisome proliferator
Exp Cell Res
(1987) - et al.
Maintenance of cytochrome P-450 and metabolism of aflatoxin B1 in primary hepatocyte cultures
Biochem Biophys Res Commun
(1977) The maintenance of cytochrome P-450 in rat hepatocyte culture: some applications of liver cell cultures to the study of drug metabolism, toxicity and the induction of the P-450 system
Chem Biol Interact
(1990)- et al.
Immunocytochemical evidence for the maintenance of cytochrome P-450 isoenzymes NADPH cytochrome C reductase and epoxide hydratase in pure and mixed primary cultures of adult human hepatocytes
Food Chem Toxicol
(1986) - et al.
Phase I and Phase II xenobiotic biotransformation in cultures and co-cultures of adult rat hepatocytes
Biochem Pharmacol
(1990)
Ethoxy-, pentoxy- and benzyloxyphenoxazones and homologues: a series of substrates to distinguish between different induced cytochromes P-450
Biochem Pharmacol
Glutathione S-transferases. The first enzymatic step in mercapturic acid formation
J Biol Chem
The carbon monoxide binding pigment of liver microsomes. I. Evidence for its hemoprotein nature
J Biol Chem
7-Ethoxycoumarin deethylase activity as a convenient measure of liver drug metabolizing enzymes: regulation in cultured rat hepatocytes
Biochem Pharmacol
Assays for differentiation of glutathione s-transferases
Methods Enzymol
A fluorometric method for determination of oxidized and reduced glutathione in tissues
Anal Biochem
Maintenance and reversibility of actice albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type
Exp Cell Res
Gastroenterology
Cited by (36)
Assessment of biliary clearance in early drug discovery using sandwich-cultured hepatocyte model
2012, Journal of Pharmaceutical SciencesCitation Excerpt :The present work has successfully expanded the study by focusing on the interplay of CLb and CYP-related metabolic activity using a collection of discovery compounds from diverse chemical scaffolds (Fig. 1). Although it has been reported that long-term cultured hepatocytes lose both phase I and phase II enzyme activity,35 and phase I metabolic activities deteriorate significantly in SCH,7 the data presented here suggests that some CYP activity remains in SCH (Fig. 6). For example, SCRH clearance values for the CYP3A substrate testosterone and the CYP1A substrate phenacetin are comparable to the clearance values in cryopreserved rat hepatocyte suspension.
Characterization of biotransformation enzyme activities in primary rat proximal tubular cells
2001, Chemico-Biological InteractionsCitation Excerpt :In contrast, our results illustrate also that phase II biotransformation levels remain more stable than phase I biotransformation levels during primary PT cell culture. Similar observations have been reported previously for GST and 1-naphthol-UGT activity in rat hepatocyte cultures [51,52]. In this study, we present the same observation for β-lyase activity.
Self-organization of liver constitutive cells mediated by artificial matrix and improvement of liver functions in long-term culture
2001, Biochemical Engineering JournalEffect of ethanol on the expression of hepatic glutathione S- transferase: An in vivo/i n vitro study
2000, Biochemical PharmacologyEnhanced cell aggregation and liver functions using polymers modified with a cell-specific ligand in primary hepatocyte cultures
1999, Journal of Bioscience and Bioengineering