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Evaluation of chromatographic methods for the determination of nifedipine in human serum

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Abstract

Gas-liquid chromatography and high-performance liquid chromatography were compared for the identification and determination of nifedipine in biological samples and the elaboration of the optimum liquid-liquid extraction procedure. The determination limits were 2 and 10 ng/ml, respectively, and the detection limits were 1 and 5 ng/ml, respectively. The calibration graphs were linear in the ranges 2–300 and 10–500 ng/ml, respectively. Recoveries based on three different concentrations were 88.7–95.8% and 93.7–104.2%, respectively. Both methods are sensitive, specific and reproducible enough for pharmocokinetic studies and therapeutic drug monitoring.

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