The isolation and purification of human peripheral blood monocytes in cell suspension

doi:10.1016/0022-1759(80)90295-1

Journal of Immunological Methods

Volume 39, Issues 1–2, 12 December 1980, Pages 71-80

https://doi.org/10.1016/0022-1759(80)90295-1 Get rights and content

Abstract

A simple method for the isolation of human peripheral blood monocytes in cell suspension is described. The method is based on the adherence and detachment characteristics of monocytes during their in vitro cultivation and does not require any gradient centrifugation or modulating substances. The mean percentage of monocytes recovered from different donors was 81.9% (60–100%) of the original phagocytic cells found in the peripheral blood of the tested donors and the cell suspensions obtained consisted of more than 95% of monocytes. The monocytes isolated in suspensions show high activity in various monocyte functions tested.

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Transforming growth factor-β is involved in the pathogenesis of dialysis-related amyloidosis.
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We have developed a rapid and simple method for isolating human peripheral blood monocytes in suspension. The procedure combines two separation media and involves isolation of the mononuclear cells by centrifugation through Ficoll-Hypaque followed by purification of the monocytes using Sepracell-MN, a colloidal silica-based medium. The final cell population contained approximately 90% monocytes with good functional ability. The contaminating cells were lymphocytes. Viability was always $≧ 99%$ with 90% recovery of the monocytes from the mononuclear cells.
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^☆: This work was supported by research grants from the Binational United States—Israel Science Foundation and the Israel Ministry of Health.

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The isolation and purification of human peripheral blood monocytes in cell suspension☆

Abstract

Prostaglandins

J. Immunol. Methods

J. Immunol. Methods

Cell. Immunol.

J. Immunol.

J. Exp. Med.

Scand. J. Immunol.

Scand. J. Immunol.

J. Exp. Med.