Elsevier

Neuropharmacology

Volume 12, Issue 9, September 1973, Pages 867-873
Neuropharmacology

On the measurement of extracellular space in slices prepared from different rat brain areas

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Abstract

Extracellular space values were determined with either 14C-sucrose or 14C-inulin at different incubation time intervals (30 and 60 min) in rat brain slices prepared from the cerebellum, cortex, striatum and hypothalamus. At both time intervals and in all brain area slices, 14C-sucrose spaces were larger than 14C-inulin spaces. Efflux of 14C-sucrose but not of inulin could be resolved into two linear components with half-times of 1.6 and 15.0 min. Efflux of 22Na from each of the different brain areas also could be separated into two distinct components, the slower of which was increased by ouabain. Similarities between 22Na and 14C-sucrose movements indicate that there is a cellular accumulation of sucrose but not of inulin. An accurate estimation of functional extracellular space in brain slices could be derived from either the 14C-inulin space or the 14C-sucrose space corrected for cellular uptake. Values obtained range from approximately 12 ml/100 g (cortex) to 20 ml/100 g (cerebellum).

References (24)

  • P.J. Goodford et al.

    The extracellular space of the smooth muscle of the guinea-pig taenia coli

    J. Physiol.

    (1966)
  • F.R. Goodman et al.

    Effects of lanthanum on 45Ca movements and on contractions induced by norepinephrine, histamine and potassium in vascular smooth muscle

    J. Pharmac. exp. Ther.

    (1971)
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