Rapid communication
The hydrolysis of oestrone sulphate and dehydroepiandrosterone sulphate by human steroid sulphatase expressed in transfected COS-1 cells

https://doi.org/10.1016/0960-0760(94)90177-5Get rights and content

Abstract

Whether the same or distinct steroid sulphatases (STS) are involved in the hydrolysis of alkyl and aryl steroid sulphates remains controversial. We have examined the ability of a placental steroid sulphatase to hydrolyse oestrone sulphate and/or dehydroepiandrosterone sulphate (DHA-S) by expressing the enzyme in COS-1 cells. Using either intact cells or broken cell preparations, the expressed sulphatase was found to hydrolyse both oestrone sulphate and DHA-S. The catalysis of oestrone sulphate and DHA-S by the expressed sulphatase was almost completely abolished by the steroid sulphatase inhibitor, oestrone-3-O-sulphamate. It is concluded that both alkyl and aryl steroid sulphates can be hydrolysed by the same steroid sulphatase.

References (21)

There are more references available in the full text version of this article.

Cited by (51)

  • Conjugated estrogens in the endometrium during the estrous cycle in pigs

    2018, Reproductive Biology
    Citation Excerpt :

    The transport of sulphated estrogens across the membrane of tissue cells is carried out by various membrane transporters of the solute carrier family (SLC) and the ATP-binding cassette transporter family (ABC) [16–19]. The steroid sulphatase (STS) is, in turn, able to catalyze the hydrolysis of the sulphate moiety resulting in the formation of free estrogens [20]. This process of reversible estrogen sulphation and desulphation is proposed to be an endogenous regulatory mechanism that enables the availability of biologically active estrogen in target tissues [21].

  • Δ<sup>4</sup>-3-ketosteroids as a new class of substrates for the cytosolic sulfotransferases

    2017, Biochimica et Biophysica Acta - General Subjects
    Citation Excerpt :

    These latter enzymes play an important role in the metabolism of steroids in the body [11]. For example, DHEA sulfate (DHEAS), produced primarily under the action of SULT2A1, is the most abundant adrenal steroids in circulation, and when transported to peripheral tissues it may be converted to DHEA by steroid sulfatase (STS) [12–13]. DHEA serves as a principal precursor for the synthesis of downstream sex steroids such as 17β-estradiol and testosterone [14].

  • Estrogen O-sulfamates and their analogues: Clinical steroid sulfatase inhibitors with broad potential

    2015, Journal of Steroid Biochemistry and Molecular Biology
    Citation Excerpt :

    Also, androstenediol is formed from androstenediol sulfate, itself derived from dehydroepiandrosterone sulfate (DHEAS) and both of these are substrates for STS. STS was found to hydrolyze readily both DHEAS and estrone sulphate, thus indicating that only one sulfatase exists for both estrogen and androgen pathways [10]. Thus, inhibition of STS should not only affect the in situ generation of estrone and estradiol, as above, but also should block the other pathway of estrogenic stimulation via androstenediol, both pathways importantly being independent of aromatase inhibition.

  • Steroid sulphatase inhibitors for breast cancer therapy

    2003, Journal of Steroid Biochemistry and Molecular Biology
View all citing articles on Scopus
View full text