Research paperDrug metabolism in hepatocyte sandwich cultures of rats and humans☆
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2019, Journal of Bioscience and BioengineeringExtra collagen overlay prolongs the differentiated phenotype in sandwich-cultured rat hepatocytes
2018, Journal of Pharmacological and Toxicological MethodsCitation Excerpt :Dedifferentiation of rat hepatocytes maintained in sandwich configuration occurs after 4 days resulting in a limited period of time during which these cultures can be used (LeCluyse et al., 1996; LeCluyse, Witek, Andersen, & Powers, 2012). Consequently, numerous optimization strategies for SCRH have been investigated (e.g. seeding density, culture media and ECM compositions) to enhance the expression and functionality of transport proteins and metabolizing enzymes, and to establish a longer maintenance of hepatocytes in culture (Annaert, Turncliff, Booth, Thakker, & Brouwer, 2001; De Bruyn et al., 2013; Kern, Bader, Pichlmayr, & Sewing, 1997). In fact, Tuschl et al. have shown preservation of the liver-specific functions of SCRH for 10 days when incubating with serum-free medium and such optimizing the sandwich model.
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This research was supported in part by the BGVV ZEBET (1328-131) and by the BMBF (BEO 21/11263).