Original ArticlesEffect of Curcumin on the Aryl Hydrocarbon Receptor and Cytochrome P450 1A1 in MCF-7 Human Breast Carcinoma Cells
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Materials
RPMI 1640, glutamine, fetal bovine serum, trypsin/EDTA, PBS, and TBE buffer were obtained from BioFluids. Curcumin, DMBA, HEPES, EDTA, dithiothreitol (DTT), glycerol, poly(dI-dC), sodium molybdate, ethyoxyresorufin, resorufin, Tris–HCl, sulforhodamine, salmon sperm, and protease inhibitors were from the Sigma Chemical Co. α-Naphthoflavone was from Indofine. [32P]CTP and [32P]dATP were from DuPont/NEN. The RT–PCR kit was from Stratagene. TBE gels, TBE running buffer, and high-density sample
Effect of Curcumin on CYP1A1 mRNA
We measured CYP1A1 mRNA in MCF-7 cells treated with DMBA in the presence or absence of curcumin using RT–PCR. Treatment of cells with 1 μM of DMBA for 24 hr caused a 15-fold increase in CYP1A1 mRNA accumulation (Fig. 1). Curcumin at 5 μM of partially inhibited the DMBA-induced increase. Surprisingly, treatment with curcumin alone caused an approximately six-fold increase in CYP1A1 mRNA. We therefore examined the effect of curcumin in the absence of other treatment on CYP1A1 mRNA. Treatment
Discussion
We examined the effect of curcumin on the AhR and the major carcinogen-activating enzyme in MCF-7 cells, CYP1A1 [25]. Using RT–PCR, we found that the mammary carcinogen DMBA caused an increase in CYP1A1 mRNA in MCF-7 cells that was partially antagonized by simultaneous treatment of the cells with curcumin (Fig. 1). It has been shown previously that the ability of the liganded AhR to induce transcription of genes depends on its ability to bind enhancer sequences, called the XRE, flanking the
Acknowledgements
The authors would like to thank Dr. Miriam Poirier for her critical reading of the manuscript.
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