Original articlesProduction of human UDP-glucuronosyltransferases 1A6 and 1A9 using the Semliki Forest virus expression system
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Semliki Forest virus vectors for rapid and high-level expression of integral membrane proteins
2003, Biochimica et Biophysica Acta - BiomembranesCitation Excerpt :For instance, cytoplasmic expression of bacterial β-galactosidase resulted in yields of 109 molecules per cell and the recombinant protein represented approximately 25% of the total cellular protein yields. Other intracellular proteins, which have resulted in high expression levels, are dehydrofolate reductase [11], thyroid peroxidase [13] and UDP-gluconoryltransferase [14]. Also a few secretory proteins have been expressed, such as plasminogen activator inhibitor (PAI) [15], interleukin-12 [16] and secreted alkaline phosphate [17].
Expression and characterization of recombinant human UDP-glucuronosyltransferases (UGTs): UGT1A9 is more resistant to detergent inhibition than the other UGTs and was purified as an active dimeric enzyme
2003, Journal of Biological ChemistryCitation Excerpt :Triton X-100 was purchased from BDH; dodecyl maltoside was from Anatrace, and phospholipids (phosphatidylcholine type X-E) were from Sigma. The human UGTs 1A6 and 1A9 were previously cloned and expressed in our laboratory (25). Complementary DNAs corresponding to 1A1, 1A3, 1A4, 1A6, 1A9, 2B4, 2B7, and 2B15 were amplified by reverse transcriptase-PCR using total liver RNA (Invitrogen) as template, a Moloney murine leukemia virus-reverse transcriptase (Sigma) for the first strand DNA synthesis, and eitherTaq DNA polymerase (Fermentas), Vent DNA polymerase (New England Biolabs), or Herculase blend (Stratagene) for the amplification.
Virus-based vectors for gene expression in mammalian cells: Semliki Forest virus
2003, New Comprehensive BiochemistryMolecular biology
2021, A Handbook of Bioanalysis and Drug MetabolismCatalytic activities of recombinant human uridine 5′-diphosphate glucuronosyltransferases 1A6 on glucuronidation of phenols and carboxylic compounds
2006, Chinese Journal of Pharmacology and Toxicology