Development of enantioselective gas chromatographic quantitation assay for dl-threo-methylphenidate in biological fluids

doi:10.1016/S0378-4347(00)80704-5

Journal of Chromatography B: Biomedical Sciences and Applications

Volume 378, 1986, Pages 109-123

https://doi.org/10.1016/S0378-4347(00)80704-5 Get rights and content

Abstract

An enantioselective gas chromatographic quantitation assay was developed for the enantiomers of dl-threo-methylphenidate in plasma and urine. dl-threo-Methylphenidate and the internal standard were acylated with N-heptafluorobutyryl-1-prolylchloride under Schotten—Baumann conditions prior to gas chromatographic separation on achiral mixed stationary phases. The derivatives were detected by means of a nitrogen—phosphorus detector. Linear and reproducible calibration curves were obtained over the concentration ranges 0.43–43.25 and 2.16–216.25 ng/ml enantiomer in plasma or urine, respectively. This enantioselective gas chromatographic quantitation assay was applied in a single oral dose disposition study of dl-threo-methylphenidate in a healthy adult volunteer. Stereoselective differences were observed in the plasma concentration—time profiles and cumulative urinary excretion profiles following oral doses of 20 and 40 mg of dl-threo-methylphenidate hydrochloride. Only d-threo-methylphenidate was detectable in plasma after 4 h.

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Citation Excerpt :
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Development of enantioselective gas chromatographic quantitation assay for dl-threo-methylphenidate in biological fluids

Abstract

J. Pharm. Sci.

Clin. Biochem.

J. Chromatogr.

J. Biol. Chem.

J. Biol. Chem.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

Biochem. Biophys. Res. Commun.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

Am. J. Orthopsychiatry