Biochemical and Biophysical Research Communications
Resveratrol scavenges reactive oxygen species and effects radical-induced cellular responses
Section snippets
Materials and methods
Materials. Resveratrol, glutathione reductase (GSSG-R), nicotinamide adenine dinucleotide phosphate reduced form (NADPH), FeSO4, H2O2, xanthine, xanthine oxidase, sodium formate, sodium dichromate (Na2Cr2O7), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin were purchased from Sigma (St. Louis, MO). 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) was purchased from Aldrich (Milwaukee, WI). Phosphate-buffered saline (PBS) was purchased from Gibco-BRL
Scavenging of by resveratrol
The Fenton reaction (Fe2+ + H2O2 → Fe3+ + + OH−) was used as a source of radicals. The results of scavenging radicals generated with this reaction by the addition of resveratrol are shown in Fig. 2. Fig. 2A shows a spectrum of an aqueous solution containing Fe2+, H2O2, and the spin trap DMPO in a phosphate-buffered saline solution. The spectrum is a 1:2:2:1 quartet with splittings at aN=aH=14.9 gauss (G). These splitting constants and the 1:2:2:1 quartet are indicative of the DMPO/ adduct [40]
Discussion
ROS and free radicals are involved in a variety of diseases and cellular response pathways. Organisms generate antioxidants, such as catalase, SOD, and uric acid, which act as endogenous antioxidant defenses. Recently, exogenous antioxidants introduced through diet or by other means are becoming popular. There has been a growing interest in the identification of possible dietary antioxidants to treat or prevent diseases caused by free radicals. These substances include fruits, vegetables, and
Acknowledgements
The authors thank Dr. James Antonini for his critical reading of the manuscript.
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