Biochemical and Biophysical Research Communications
Novel single nucleotide polymorphisms of organic cation transporter 1 (SLC22A1) affecting transport functions
Section snippets
Materials and methods
Materials. [14C]TEA (2.05 GBq/mmol) and [3H]MPP+ (2.96 TBq/mmol) were purchased from American Radiolabeled Chemicals (USA). All other chemicals and reagents were of analytical grade. Rabbit polyclonal antibodies were raised against a synthetic carboxyl-terminal peptide of human OCT1, ENLGRKAKPKENC, as reported previously [14].
Isolation of human OCT1 cDNA. Specific PCR primers were designed based on the nucleotide sequence for human OCT1 (GenBank Accession Nos. U77086 and NM_003057) [9]: forward
Polymorphisms in OCT1 in Japanese population
In JSNP database, we found four nonsynonymous nucleotide polymorphisms of OCT1: F160L (JSNP ID: ssj0008476), P283L (ssj0005319), R287G (ssj0005320), and P341L (ssj0008480). SNPs F160L and P341L were same as those reported previously for American population [11], [12], while SNPs P283L and R287G are only found in Japanese population. As shown in Fig. 1, F160 is located in the middle of the second transmembrane domain (TMD): P283 and R287 are at the 5′-end of long intracellular loop between 6th
Acknowledgements
The anti-hOCT1 polyclonal antibody was supplied by Transgenic Inc. Kumamoto, Japan. This work was supported in part by grants from the Ministry of Education Science, Sports, Culture and Technology of Japan, and from the Japanese Society for the Promotion of Science (JSPS). This work was performed in collaboration with PharmaSNP consortium.
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