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Detection of P-glycoprotein in multidrug-resistant cell lines by monoclonal antibodies

Nature volume 316, pages 820–823 (1985)Cite this article

Abstract

One reason for the failure of chemotherapy in the treatment of advanced cancers may be the outgrowth of multidrug-resistant tumour cells1–5. Multidrug resistance has been modelled in numerous mammalian cell lines in which the phenotype is characterized by a pleiotropic cross-resistance to unrelated drugs1–7. In the study reported here, we have produced monoclonal antibodies whose binding to plasma membranes of different multidrug-resistant mammalian cells correlates with the degree of drug resistance. All these antibodies are specific for P-glycoprotein, a cell surface component of relative molecular mass (Mr) 170,000 (170K) that has been described previously8–15, and are directed against three spatially distinct epitopes which define a conserved cytoplasmic domain in the C-terminal region of the P-glycoprotein polypeptide. The conserved nature of P-glycoprotein and its low-level expression in drug-sensitive cells suggest that it has an important function at the cell surface. The monoclonal antibodies against P-glycoprotein described here might serve as diagnostic reagents for clinically unresponsive tumours16.

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Authors and Affiliations

  1. Ontario Cancer Institute, Princess Margaret Hospital,

    Norbert Kartner, Deanna Evernden-Porelle, Grace Bradley & Victor Ling

  2. Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada, M4X 1K9

    Norbert Kartner, Deanna Evernden-Porelle, Grace Bradley & Victor Ling

Authors
  1. Norbert Kartner
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  3. Grace Bradley
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  4. Victor Ling
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Kartner, N., Evernden-Porelle, D., Bradley, G. et al. Detection of P-glycoprotein in multidrug-resistant cell lines by monoclonal antibodies. Nature 316, 820–823 (1985). https://doi.org/10.1038/316820a0

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