17β-estradiol and anti-estrogen ICI:Compound 182,780 regulate expression of lipoprotein lipase and hormone-sensitive lipase in isolated subcutaneous abdominal adipocytes*,**
Section snippets
Subjects
SC abdominal adipose tissue was obtained from 17 women undergoing elective abdominal surgery, with the majority of cases being performed for cosmetic reasons. The mean age for the group was 47 years (range, 34 to 62), mean weight was 65.4 kg (range, 58.1 to 75.0), and mean body mass index (BMI) was 25 kg/m2 (range, 22 to 27). Subjects using endocrine therapy such as corticosteroids, thyroxine, and either the oral contraceptive pill or HRT were excluded, as were those on lipid-lowering
Effect of E2
Increasing concentrations of E2 altered LPL protein expression relative to control, with the highest dose (E210−7 mol/L) significantly reducing LPL protein expression and the lower doses increasing LPL protein expression; mean protein expression relative to control ± SE: E2 10−12 mol/L, 1.79 ± 0.16 (P <.001); E2 10−7 mol/L, 0.56 ± 0.08 (P <.05) (Fig 1A).
Discussion
Human epidemiological and prospective clinical studies suggest estrogen deficiency is associated with the development of central obesity, which may be limited or reversed by HRT.6, 7, 29 Our findings provide the first in vitro evidence that E2 has a regulatory effect on key enzymes of lipogenesis and lipolysis in SC abdominal adipose tissue from women. These results suggest that E2 may regulate adipose tissue mass by influencing the net amount of adipose tissue present in the adipocyte. Taken
Acknowledgements
We thank all the surgeons and theatre staff at the University Hospitals Trust and the Priory Hospital, Edgbaston, Birmingham who aided in the studies. We especially thank Dr Allan Green of Bassett Healthcare Research Institute, Cooperstown, NY, for providing the HSL antibody.
References (40)
The degree of masculine differentiation of obesities: A factor determining predisposition to diabetes, atherosclerosis, gout and uric calculous disease
Am J Clin Nutr
(1956)- et al.
Postmenopausal hormone replacement therapy prevents central distribution of body fat after menopause
Metabolism
(1991) - et al.
Changes in fat cell size and in-vitro lipolytic activity of abdominal and gluteal adipocytes after a one-year cross-sex hormone administration in transsexuals
Metabolism
(1999) - et al.
Cellularity in different regions of adipose tissue in young men and women
Metabolism
(1972) - et al.
Effects of 2-fluoroestradiol on lipid metabolism in the ovariectomized rat
J Steroid Biochem
(1986) - et al.
Comparison of pharmacodynamic properties of various estrogen formulations
Am J Obstet Gynecol
(1982) Effects of postmenopausal hormone replacement therapy on lipid, lipoprotein, and apolipoprotein (a) concentrations: Analysis of studies published from 1974-2000
Fertil Steril
(2001)- et al.
Identification of estrogen receptor beta RNA in human breast and abdominal subcutaneous adipose tissue
Biochem Biophys Res Commun
(1998) Obesity as a medical problem
Nature
(2000)- et al.
Relationship of body fat distribution to metabolic complications of obesity
J Clin Endocrinol Metab
(1982)
Abdominal adipose tissue distribution, obesity and risk of cardiovascular disease and death: 13 year follow up of participants in the study of men born in 1913
BMJ
Menopause and risk of cardiovascular disease—The Framingham Study
Ann Intern Med
Body weight, body fat distribution, and hormonal replacement therapy in early postmenopausal women
J Clin Endocrinol Metab
Regulation of adipose cell number in man
Clin Sci
Diet-induced adipocyte number increase in adult rats: A new model of obesity
Am J Physiol
Lipoprotein lipase—A multifunctional enzyme relevant to common metabolic diseases
N Engl J Med
Adipocyte hormone-sensitive lipase: A major regulator of lipid metabolism
Proc Nutr Soc
Regional adiposity and morbidity
Physiol Rev
Promotion of human adipocyte precursor replication by 17β-estradiol in culture
J Clin Invest
Opposite effects of androgens and estrogens on adipogenesis in rat preadipocytes: Evidence for sex and site-related specificities and possible involvement of insulin-like growth factor 1 receptor and peroxisome proliferator-activated receptor γ2*
Endocrinol
Cited by (93)
Sex-specific regulation of miR-22 and ERα in white adipose tissue of obese dam's female offspring impairs the early postnatal development of functional beige adipocytes in mice
2024, Biochimica et Biophysica Acta - Molecular Basis of DiseaseThe follicular fluid metabolome differs according to the endometriosis phenotype
2020, Reproductive BioMedicine OnlineGonad metabolomics and blood biochemical analysis reveal differences associated with testicular oocytes in wild largemouth bass (Micropterus salmoides)
2020, Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular BiologyCitation Excerpt :To identify these commonalities associated with oocytes, analyte directional response patterns (Table 2) increasing or decreasing in an order consistent with the level of oocytes in sexual phenotype groups (i.e. a medial [•] response of high TO individuals) serves to highlight putative metabolites of interest. Glycerol, which was elevated in females and reduced in low TO males is indicative of lipid metabolism and has been shown to be released by adipocytes following exposure to estrogen (Palin et al., 2003). Of note, the putative metabolites glycine, alanine, and pyroglutamic acid were lowest in females and highest in low TO males.
Endometriosis phenotypes are associated with specific serum metabolic profiles determined by proton-nuclear magnetic resonance
2020, Reproductive BioMedicine OnlineCitation Excerpt :Indeed, the OMA samples had significantly higher concentrations of two ketone bodies (acetone and 3HB), whereas the DIE patients had higher concentrations of lipids. One explanation could be that the oestradiol concentration seems to be higher in OMA compared with DIE (Huhtinen et al., 2012), which could increase lipase activity, as previously shown by Palin et al. (2003) and Cox-York et al. (2017). Lipolysis would, therefore, be enhanced in OMA compared with DIE, thereby resulting in higher ketone body levels in OMA samples, compared with higher lipid levels in DIE samples, as observed in our study.
Regulation of triglyceride synthesis by estradiol in the livers of hybrid tilapia (Oreochromis niloticus ♀ × O. aureus ♂)
2019, Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
- *
Address reprint requests to Dr S.L. Palin, Diabetes Centre (Ward 34), Birmingham Heartlands Hospital, Bordesley Green East, Birmingham B9 5SS, UK.
- **
0026-0495/03/5204-0027$30.00/0