Analysis of Cardioprotective Effects Using Purified Salvia miltiorrhiza Extract on Isolated Rat Hearts

Piek Ngoh Chang; Jian Chun Mao; Shan Hong Huang; Li Ning; Zhong Jing Wang; Todd On; Wei Duan; Yi Zhun Zhu

doi:10.1254/jphs.FPJ05034X

Full Papers

Analysis of Cardioprotective Effects Using Purified Salvia miltiorrhiza Extract on Isolated Rat Hearts

Piek Ngoh Chang, Jian Chun Mao, Shan Hong Huang, Li Ning, Zhong Jing Wang, Todd On, Wei Duan, Yi Zhun Zhu

Author information

Piek Ngoh Chang
Department of Pharmacology, Faculty of Medicine, National University of Singapore, Singapore
Jian Chun Mao
Department of Internal Medicne, Long Hua Hospital, Shanghai University of Traditional Chinese Medicine, China
Shan Hong Huang
Department of Biochemistry, Faculty of Medicine, National University of Singapore, Singapore
Li Ning
Department of Pharmacology, Faculty of Medicine, National University of Singapore, Singapore
Zhong Jing Wang
Department of Pharmacology, Faculty of Medicine, National University of Singapore, Singapore
Todd On
Department of Pharmacology, Faculty of Medicine, National University of Singapore, Singapore
Wei Duan
Department of Biochemistry, Faculty of Medicine, National University of Singapore, Singapore
Yi Zhun Zhu
Department of Pharmacology, Faculty of Medicine, National University of Singapore, Singapore
School of Pharmacy and Institute of Biomedical Sciences, Fudan University, China

Keywords: purified Salvia miltiorrhiza extract, cardioprotective effect, myocardial ischemia/reperfusion, isolated rat heart

JOURNAL FREE ACCESS

2006 Volume 101 Issue 3 Pages 245-249

DOI https://doi.org/10.1254/jphs.FPJ05034X

View "Advance Publication" version (July 13, 2006).

Details

Abstract

The purpose of the current study is to evaluate the cardioprotective effects of purified Salvia miltiorrhiza extract (PSME) on myocardial ischemia/reperfusion injury in isolated rat hearts. Hearts were excised and perfused at constant flow (7 – 9 ml · min⁻¹) via the aorta. Non-recirculating perfusion with Krebs-Henseleit (KH) solution was maintained at 37°C and continuously gassed with 95% O₂ and 5% CO₂. KH solution with or without PSME (100 mg per liter solution) was used after 30-min zero-flow ischemia for the PSME and control group, respectively. Left ventricular (LV) developed pressure; its derivatives, diastolic pressure, and so on were continuously recorded via a pressure transducer attached to a polyvinylchloride balloon that was placed in the left ventricle through an incision in the left atrium. PSME treated hearts showed significant postischemic contractile function recovery (developed pressure recovered to 44.2 ± 4.9% versus 17.1 ± 5.7%, P<0.05; maximum contraction recovered to 57.2 ± 5.9% versus 15.1 ± 6.3%, P<0.001; maximum relaxation restored to 69.3 ± 7.3% versus 15.4 ± 6.3%, P<0.001 in the PSME and control group, respectively). Significant elevation in end-diastolic pressure, which indicated LV stiffening in PSME hearts might have resulted from the excess high dose of PSME used. Further study will be conducted on the potential therapeutic value with lower dose of PSME on prevention of ischemic heart disease.

Corresponding author

Register with J-STAGE for free!