Abstract
To isolate the murine Na+/taurocholate cotransporting polypeptide (Ntcp), we screened a mouse liver cDNA library and identified Ntcp1, encoding a 362 amino acid protein and Ntcp2, encoding a 317 amino acid protein which had a shorter C-terminal end. Both isoforms mediated saturable Na+-dependent transport of taurocholate when expressed in Xenopus laevis oocytes. Analysis of the gene revealed that Ntcp2 is produced by alternative splicing where the last intron is retained.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Base Sequence
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Carrier Proteins / genetics*
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Cloning, Molecular
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Gene Library
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Liver / metabolism*
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Membrane Transport Proteins*
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Mice
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Molecular Sequence Data
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Oocytes / metabolism
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Organic Anion Transporters, Sodium-Dependent
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Protein Isoforms / genetics
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Sequence Alignment
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Symporters
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Xenopus laevis
Substances
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Carrier Proteins
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Membrane Transport Proteins
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Organic Anion Transporters, Sodium-Dependent
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Protein Isoforms
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Symporters
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sodium-bile acid cotransporter
Associated data
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GENBANK/U95131
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GENBANK/U95132