Assignment of overlapping (1)H NMR signals in carp seminal plasma by proton-detected 2D C,H correlation spectroscopy

Biochem Biophys Res Commun. 2000 Mar 5;269(1):76-80. doi: 10.1006/bbrc.2000.2250.

Abstract

The (1)H NMR spectrum of the perchloric acid extract of carp seminal plasma was heavily congested. It is demonstrated that proton-detected C,H chemical shift correlation spectroscopy (HSQC, HSQC-TOCSY) allows an unequivocal identification of proline, glutamate, taurine, and methionine sulfoxide, although several key proton signals were strongly overlapped.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carps / metabolism*
  • Glutamic Acid / chemistry
  • Magnetic Resonance Spectroscopy
  • Male
  • Methionine / analogs & derivatives
  • Methionine / chemistry
  • Proline / chemistry
  • Protons
  • Semen / chemistry*
  • Spectrum Analysis / methods
  • Taurine / chemistry

Substances

  • Protons
  • Taurine
  • Glutamic Acid
  • Proline
  • Methionine
  • methionine sulfoxide