Abstract
The imaging of cellular dynamics in three dimensions using a standard microscope is severely limited due to the fact that only one focal plane can be imaged at a given point in time. Here we present a modification of the classical microscope design with which two or more focal planes can be imaged simultaneously. This is achieved by a modification of the emission pathway of a standard microscope. The efficacy of the design is shown by imaging bead samples and an FcRn-green fluorescent protein expressing tubule that leaves a sorting endosome and subsequently exocytoses at the plasma membrane.
Publication types
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Evaluation Study
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Research Support, U.S. Gov't, P.H.S.
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Validation Study
MeSH terms
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Cell Line
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Endothelial Cells / cytology*
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Endothelial Cells / metabolism*
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Equipment Design
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Equipment Failure Analysis
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Histocompatibility Antigens Class I
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Humans
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Image Enhancement / instrumentation*
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Imaging, Three-Dimensional / instrumentation*
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Imaging, Three-Dimensional / methods
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Microscopy, Fluorescence / instrumentation*
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Microscopy, Fluorescence / methods
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Microscopy, Video / instrumentation*
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Microscopy, Video / methods
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Receptors, Fc / metabolism*
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Reproducibility of Results
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Sensitivity and Specificity
Substances
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Histocompatibility Antigens Class I
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Receptors, Fc
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Fc receptor, neonatal