Evidence for CEA release from human colon cancer cells by an endogenous GPI-PLD enzyme

Fakhraddin Naghibalhossaini; Padideh Ebadi

doi:10.1016/j.canlet.2005.03.028

Evidence for CEA release from human colon cancer cells by an endogenous GPI-PLD enzyme

Cancer Lett. 2006 Mar 28;234(2):158-67. doi: 10.1016/j.canlet.2005.03.028.

Authors

Fakhraddin Naghibalhossaini¹, Padideh Ebadi

Affiliation

¹ Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, 71345 Zand Street, P.O. Box 1167 Shiraz, Iran. fakhraddin.naghibalhossaini@elf,mcgill.ca

PMID: 15893415
DOI: 10.1016/j.canlet.2005.03.028

Abstract

Elevated carcinoembryonic antigen (CEA) blood levels are found in a wide variety of epithelial neoplasms. The precise mechanism of the spontaneous CEA release from normal and cancer cells has not been established yet. In this study we investigated 'in vitro' the role of an endogenous glycosylphosphatidyl inositol phospholipase D (GPI-PLD) in spontaneous CEA release from human colon carcinoma cells. We detected GPI-PLD-specific transcript expression in four human colorectal tumor cell lines, LS180, HT29, HT29/219, and SW742 by RT-PCR. Furthermore, CEA release could be activated and inhibited by incubation of LS180 cells with suramin and 1,10-phenanthroline, compounds known to activate and inhibit GPI-PLD activity, respectively. The results suggest a mechanism for the involvement of an endogenous GPI-PLD in spontaneous CEA release from human colon cancer cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinoembryonic Antigen / drug effects
Carcinoembryonic Antigen / metabolism*
Cell Line, Tumor
Colonic Neoplasms / metabolism*
Enzyme Inhibitors / pharmacology
Humans
Phospholipase D / biosynthesis*
Phospholipase D / drug effects
Reverse Transcriptase Polymerase Chain Reaction

Substances

Carcinoembryonic Antigen
Enzyme Inhibitors
Phospholipase D
glycoprotein phospholipase D