cDNA and deduced amino acid sequences of a dog liver cytochrome P-450 of the IIIA gene subfamily

Biochim Biophys Acta. 1991 Feb 16;1088(2):319-22. doi: 10.1016/0167-4781(91)90072-t.

Abstract

A 1.96 kbp cDNA encoding a male Beagle dog liver cytochrome P-450 of 503 amino acid residues (Mr 57,636) has been isolated and sequenced. The deduced amino acid sequence is 79.8%, 69.3% and 74.1% identical to the P450IIIA forms human NF25, rat PCN1 and rabbit LM3c, respectively. The amino terminal sequence is identical to the first 28 residues of the dog P450IIIA form PBD-1. Southern blot analysis yields restriction patterns consistent with IIIA gene subfamily multiplicity.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cytochrome P-450 Enzyme System / genetics*
  • DNA / genetics*
  • DNA / isolation & purification
  • Dogs
  • Humans
  • Liver / enzymology*
  • Male
  • Molecular Sequence Data
  • Molecular Weight
  • Multigene Family*
  • Rabbits
  • Sequence Homology, Nucleic Acid

Substances

  • DNA
  • Cytochrome P-450 Enzyme System

Associated data

  • GENBANK/M75971
  • GENBANK/M75972
  • GENBANK/M75973
  • GENBANK/M75974
  • GENBANK/M75975
  • GENBANK/S57579
  • GENBANK/S59357
  • GENBANK/X54747
  • GENBANK/X54748
  • GENBANK/X54915