Nitidine Chloride inhibits breast cancer cells migration and invasion by suppressing c-Src/FAK associated signaling pathway

Xinhua Pan; Honghui Han; Lei Wang; Linli Yang; Ruimei Li; Zhenxi Li; Junchen Liu; Qufei Zhao; Min Qian; Mingyao Liu; Bing Du

doi:10.1016/j.canlet.2011.09.001

Nitidine Chloride inhibits breast cancer cells migration and invasion by suppressing c-Src/FAK associated signaling pathway

Cancer Lett. 2011 Dec 27;313(2):181-91. doi: 10.1016/j.canlet.2011.09.001. Epub 2011 Sep 10.

Authors

Xinhua Pan¹, Honghui Han, Lei Wang, Linli Yang, Ruimei Li, Zhenxi Li, Junchen Liu, Qufei Zhao, Min Qian, Mingyao Liu, Bing Du

Affiliation

¹ The Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.

PMID: 21959111
DOI: 10.1016/j.canlet.2011.09.001

Abstract

Nitidine is a benzophenanthridine alkaloid, which has been shown to have anti-tumor properties. Here, we demonstrated that Nitidine Chloride (NC) could inhibit breast cancer cells migration and invasion both in vitro and in vivo. Meanwhile, the protrusion formation and partial proteolytic activity of MMP-9 and MMP-2 were attenuated by NC in a dose-dependent manner in MDA-MB-231 cells. Furthermore, addition NC to cells significantly decreases PDGF induced phosphorylation of c-Src, FAK, MAPKs, activation of RhoA, Rac1 and AP-1 transcriptional activity. Taken together, our results indicate that NC could have potential as a novel anti-metastasis drug to breast cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents, Phytogenic / pharmacology
Benzophenanthridines / pharmacology*
Breast Neoplasms / drug therapy*
Breast Neoplasms / metabolism
Breast Neoplasms / pathology*
CSK Tyrosine-Protein Kinase
Cell Line, Tumor
Cell Movement / drug effects
Enzyme Activation / drug effects
Extracellular Signal-Regulated MAP Kinases / metabolism
Female
Focal Adhesion Kinase 1 / metabolism
Humans
Matrix Metalloproteinase 2 / metabolism
Matrix Metalloproteinase 9 / metabolism
Mice
Mice, Nude
Neoplasm Invasiveness
Platelet-Derived Growth Factor / toxicity
Protein-Tyrosine Kinases / metabolism
Signal Transduction / drug effects
Transcription Factor AP-1 / genetics
Transcription Factor AP-1 / metabolism
rac1 GTP-Binding Protein / metabolism
rhoA GTP-Binding Protein / metabolism
src-Family Kinases

Substances

Antineoplastic Agents, Phytogenic
Benzophenanthridines
Platelet-Derived Growth Factor
RAC1 protein, human
Transcription Factor AP-1
nitidine
Protein-Tyrosine Kinases
CSK Tyrosine-Protein Kinase
Focal Adhesion Kinase 1
PTK2 protein, human
src-Family Kinases
CSK protein, human
Extracellular Signal-Regulated MAP Kinases
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
rac1 GTP-Binding Protein
rhoA GTP-Binding Protein